Biodegradability of commercial and weathered diesel oils

Brazilian Journal of Microbiology

Adriano Pinto Mariano²  Daniel Marcos Bonotto²  Dejanira De Franceschi De Angelis¹  Maria Paula Santos Pirôllo¹  Jonas Contiero¹ 

[1] ,Universidade Estadual Paulista Instituto de Geociências e Ciências Exatas Rio Claro SP ,Brasil

关键词: biodegradability;    bioremediation;    commercial oil diesel;    weathered diesel oil;    biodegradabilidade;    biorremediação;    óleo diesel comercial;    óleo diesel intemperizado;   

DOI  :  10.1590/S1517-83822008000100028

来源: SciELO

【 摘 要 】

This work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. Two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 - dichlorophenol indophenol (DCPIP) and soil respirometric experiments using biometer flasks. In the former we tested the bacterial cultures Staphylococcus hominis, Kocuria palustris, Pseudomonas aeruginosa LBI, Ochrobactrum anthropi and Bacillus cereus, a commercial inoculum, consortia obtained from soil and groundwater contaminated with hydrocarbons and a consortium from an uncontaminated area. In the respirometric experiments it was evaluated the capability of the native microorganisms present in the soil from a petrol station to biodegrade the diesel oils. The redox indicator experiments showed that only the consortia, even that from an uncontaminated area, were able to biodegrade the weathered diesel. In 48 days, the removal of the total petroleum hydrocarbons (TPH) in the respirometric experiments was approximately 2.5 times greater when the commercial diesel oil was used. This difference was caused by the consumption of labile hydrocarbons, present in greater quantities in the commercial diesel oil, as demonstrated by gas chromatographic analyses. Thus, results indicate that biodegradability studies that do not consider the weathering effect of the pollutants may over estimate biodegradation rates and when the bioaugmentation is necessary, the best strategy would be that one based on injection of consortia, because even cultures with recognised capability of biodegrading hydrocarbons may fail when applied isolated.

【 授权许可】

CC BY-NC   
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