Rapid diagnosis of vesicular stomatitis virus in Ecuador by the use of polymerase chain reaction

Brazilian Journal of Microbiology

Lya Madureira Sepúlveda²  Viviana Malirat²  Ingrid E. Bergmann²  Anibal Mantilla¹  Elmiro Rosendo Do Nascimento¹ 

[1] ,OMS OPS Centro Pan-Americano de Febre AftosaRio de Janeiro RJ ,Brasil

关键词: Polymerase Chain reaction;    Vesicular Stomatitis;    molecular diagnosis;    Reação em Cadeia da Polimerase;    Estomatite Vesicular;    Diagnóstico molecular;   

DOI  :  10.1590/S1517-83822007000300022

来源: SciELO

【 摘 要 】

Vesicular Stomatitis (VS) is a viral disease that has a great impact in animal health, as infected animals present marked decrease in meat and milk production. Its presence is a limiting factor for international animal trade. Besides the damage in the livestock productivity, such disease assumes an important role in animal health programs since it is clinically indistinguishable from Foot-and-Mouth Disease. The diagnosis of the VS has been made, mainly, through Complement Fixation, ELISA and Virus Neutralization tests, assays that allow not only for viral detection but also for differentiation of the two serotypes described for Vesicular Stomatitis Virus (VSV): New Jersey (NJ) and Indiana (Ind). In this work, a molecular diagnostic approach, the polymerase chain reaction performed after reverse transcription (RT - PCR), based on the specific partial amplification of NS gene of VSV was used, as an alternative method for the detection of the virus. A total of 10 VSV reference samples and 12 specimens collected from animals with clinical signs of vesicular disease obtained from field episodes in Ecuador were tested. The method allowed for the specific partial amplification of the region coding for protein P, both for VSV serotypes New Jersey (642 bp) and Indiana 1 (614 bp). The results were compatible with data obtained by Complement Fixation test and the identity of the amplified products was confirmed by nucleotide sequencing.

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