Brazilian Journal of Microbiology | |
Comparison between ectodomain and G2 region of G glycoprotein for genotyping of HRSV | |
Angélica Cristine De Almeida Campos2  Edison Luiz Durigon2  Andréa Lima Leal2  Tereza Souza Silva2  Patrícia Alves Ramos Bosso2  Claudia Trigo Pedroso Moraes2  Danielle Bruna Leal Oliveira2  Hildener Nogueira Lima2  Sandra Elisabete Vieira1  Viviane Fongaro Botosso1  Paolo Marinho De Andrade Zanotto2  Klaus Eberhard Stewien2  | |
[1] ,Universidade de São Paulo Instituto de Ciências Biomédicas São Paulo SP ,Brasil | |
关键词: Human Respiratory Syncytial Virus; G Glycoprotein; G2 Region; Phylogenetic Analysis; Vírus Respiratório Sincicial Humano; Glicoproteína G; Região G2; Análise Filogenética; | |
DOI : 10.1590/S1517-83822007000300005 | |
来源: SciELO | |
【 摘 要 】
The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three nasal swab samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank sequences. We concluded that the G2 region is adequate for HRSV genotyping.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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