Comparison between ectodomain and G2 region of G glycoprotein for genotyping of HRSV

Brazilian Journal of Microbiology

Angélica Cristine De Almeida Campos²  Edison Luiz Durigon²  Andréa Lima Leal²  Tereza Souza Silva²  Patrícia Alves Ramos Bosso²  Claudia Trigo Pedroso Moraes²  Danielle Bruna Leal Oliveira²  Hildener Nogueira Lima²  Sandra Elisabete Vieira¹  Viviane Fongaro Botosso¹  Paolo Marinho De Andrade Zanotto²  Klaus Eberhard Stewien² 

[1] ,Universidade de São Paulo Instituto de Ciências Biomédicas São Paulo SP ,Brasil

关键词: Human Respiratory Syncytial Virus;    G Glycoprotein;    G2 Region;    Phylogenetic Analysis;    Vírus Respiratório Sincicial Humano;    Glicoproteína G;    Região G2;    Análise Filogenética;   

DOI  :  10.1590/S1517-83822007000300005

来源: SciELO

【 摘 要 】

The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three nasal swab samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank sequences. We concluded that the G2 region is adequate for HRSV genotyping.

【 授权许可】

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