Do DNA extraction methods and Taq polimerase quality improve the double repetitive element (DRE) PCR typing method for Mycobacterium tuberculosis strains?

Brazilian Journal of Microbiology

Hebe Rodrigues Cavalcanti¹  Elizabeth Marques¹  Leila De Souza Fonseca²  Maria Helena Féres Saad² 

[1] ,Universidade Estadual do Rio de Janeiro Hospital Universitário Pedro Ernesto Rio de Janeiro RJ ,Brasil

关键词: Mycobacterium tuberculosis;    PCR;    taq hot start;    DRE-PCR;    Mycobacterium tuberculosis;    PCR;    Taq hot start;    DRE-PCR;   

DOI  :  10.1590/S1517-83822007000300004

来源: SciELO

【 摘 要 】

Double repetitive element (DRE) PCR amplification is a simple Mycobacterium tuberculosis typing method, however amplification failure or poor resolution of bands commit its efficacy. In order to verify if whether or not these features could be minimized by improving DNA extraction procedures or Taq polymerise quality, DRE-PCR was performed on 24 M. tuberculosis DNA samples extracted by heat-shock, mechanical and enzymatic methods applying conventional and hot start Taq pol. We demonstrated that when dealing with the Mycobacterium tuberculosis DRE-PCR typing method, Taq pol of better quality might be more important to improve amplification than the DNA extraction method.

【 授权许可】

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