Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system

Brazilian Journal of Microbiology

Juliana A. Santos²  Marcel C.o. Amaral¹  Thiago A.s. Araújo²  Késsia G.c. Fernandes²  Adilson C. Chaves²  Márcia M.c. Morais² 

[1] ,University of Pernambuco Institute of Biological Sciences Recife Pernambuco ,Brazil

关键词: extracellular lipase;    Trichosporon;    aqueous two phase system;    PEG;    lipase extracelular;    Trichosporon;    sistema bifásico aquoso;   

DOI  :  10.1590/S1517-83822007000100013

来源: SciELO

【 摘 要 】

A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.

【 授权许可】

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