期刊论文详细信息
Brazilian Journal of Microbiology | |
Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system | |
Juliana A. Santos2  Marcel C.o. Amaral1  Thiago A.s. Araújo2  Késsia G.c. Fernandes2  Adilson C. Chaves2  Márcia M.c. Morais2  | |
[1] ,University of Pernambuco Institute of Biological Sciences Recife Pernambuco ,Brazil | |
关键词: extracellular lipase; Trichosporon; aqueous two phase system; PEG; lipase extracelular; Trichosporon; sistema bifásico aquoso; | |
DOI : 10.1590/S1517-83822007000100013 | |
来源: SciELO | |
【 摘 要 】
A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
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