Ochratoxin A in wines and grape juices commercialized in the city of São Paulo, Brazil

Brazilian Journal of Microbiology

Luzia Shundo¹  Adriana P. De Almeida¹  Janete Alaburda¹  Valter Ruvieri¹  Sandra A. Navas¹  Leda C.a. Lamardo¹  Myrna Sabino¹ 

[1] ,Instituto Adolfo Lutz Seção de Química Biológica São Paulo SP ,Brasil

关键词: Ochratoxin A;    red wine;    grape juice;    immunoaffinity colunm;    HPLC determination;    Ocratoxina A;    vinho tinto;    suco de uva;    coluna de imunoafinidade;    determinação por CLAE;   

DOI  :  10.1590/S1517-83822006000400024

来源: SciELO

【 摘 要 】

During the summer of 2005, a total of 101 samples of wines and grape juices purchased from supermarkets and retail stores in São Paulo city were analysed for the presence of Ochratoxin A (OTA). OTA was evaluated in 29 red wines and 38 grape juices produced in Brazil and in 34 imported red wines (from Argentina, Chile, Uruguay, France, Italy, Portugal, Spain and South Africa). OTA was extracted in an immunoaffinity column and detected by HPLC with fluorescence detection, according to EN 14133/2003. The detection and quantification limits established were 0.01 and 0.03 ng/mL, respectively. The recoveries for wine samples were 94.1, 82.5, 86.1% and the relative standard deviation were 6.10, 1.03, 4.11% at levels of 0.03, 2.0, 5.0 ng/mL, respectively. For grape juice, the recovery was 86.2% and the RSD was 2.01% at a level of 0.4 ng/mL. OTA contamination was found in nine of the 29 Brazilian red wines with levels ranging from 0.10 to 1.33 ng/mL and in 18 of the 34 imported red wines with levels ranging from 0.03 to 0.32 ng/mL. OTA was not detected in any of the grape juice samples analysed. Although the results from the wine samples analysed for the presence of OTA were below to the limits established by EC 123/2005 (2.0 ng/mL), low and continuous exposure to this mycotoxin could be a risk to human health.

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