Use of single-enzyme amplified fragment length polymorphism for typing Clostridium perfringens isolated from diarrheic piglets

Brazilian Journal of Microbiology

Luciane Tieko Shinya²  Maria Regina Baccaro¹  Andrea Micke Moreno² 

[1] ,Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Medicina Veterinária Preventiva e Saúde AnimalSão Paulo SP ,Brasil

关键词: AFLP;    diarrhea;    piglets;    Clostridium perfringens;    AFLP;    diarréia;    suínos;    Clostridium erfringens;   

DOI  :  10.1590/S1517-83822006000300034

来源: SciELO

【 摘 要 】

Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from swine presenting diarrhea. Analysis of the results showed 29 distinct profiles with discriminatory index equal to 0.97. Partial correlation between the origin of the isolates and groups was drawn, and correlation was possible in only 18.5% of the samples. Characterization of the strains in biotypes (A, B, C, D and E), production of beta-2 toxin and enterotoxin were performed by means of the polymerase chain reaction (PCR). Biotypes A, C and D were observed among the strains analyzed. All samples were positive for presence of the gene encoding beta-2 toxin and negative for the gene encoding enterotoxin. AFLP have shown to be a simple, fast, low cost method with high discriminative power and good reproducibility, presenting a great potential in epidemiological studies involving C. perfringens strains of animal origin.

【 授权许可】

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