Evaluation of the Diramic system for urine cultures

Brazilian Journal of Microbiology

Cristian Kendi Higuchi²  Carlos Roberto Corrêa¹  Vera Lucia Mores Rall²  Ary Fernandes Junior² 

[1] ,Estadual Paulista Julio de Mesquita Filho Instituto de Biociências Departamento de Microbiologia e ImunologiaBotucatu SP ,Brasil

关键词: Diramic;    calibrated loop;    urinary tract infection;    urine cultures;    sistema Diramic;    alça calibrada;    ITU;    urinoculturas;   

DOI  :  10.1590/S1517-83822006000300005

来源: SciELO

【 摘 要 】

The use of the Diramic system in microbiological diagnosis of urinary tract infections (UTI) was evaluated. This system was developed at the National Center for Scientific Research of Cuba, and it reads turbidimetric changes of microbial growth in culture media at 37ºC incubated for 4 hours. A total of 396 urine specimens were tested in the Laboratory of Microbiology of the School of Medicine - UNESP, Brazil using the Diramic system and the counting of colony forming units per urine millimeter (calibrated loop) as the reference method. The coincidence rate between the two methods was 96.46% (382 urine samples), and the differences in results were not significant (p<0.05). Sensitivity and specificity rates were 84.37% and 98.80%, respectively. False negative and false positive rates were 2.50% and 1.01%, respectively. The microorganisms isolated from positive urines were: Escherichia coli (68.75%); Klebsiella pneumoniae (10.94%); yeast (6.25%); Pseudomonas aeruginosa (4.69%); Enterobacter cloacae (3.12%); Proteus mirabilis, coagulase negative Staphylococcus, Morganella morganii, and Citrobacter freundii (1.56% each). The Diramic system was effective as screening method for urine cultures, however restrictions in the UTI diagnosis caused by yeasts and patients undergoing antibiotic therapy were negative characteristics of the system.

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