Partial purification of elicitors from Lentinula edodes basidiocarps protecting cucumber seedlings against Colletotrichum lagenarium

Brazilian Journal of Microbiology

Robson M. Di Piero²  Nelson A. Wulff¹  Sérgio F. Pascholati¹ 

[1] ,Universidade Federal de Santa Catarina Centro de Ciências Agrárias Departamento de FitotecniaFlorianópolis SC ,Brasil

关键词: shiitake;    anthracnose;    peroxidase;    shiitake;    antracnose;    peroxidases;   

DOI  :  10.1590/S1517-83822006000200015

来源: SciELO

【 摘 要 】

The shiitake mushroom (Lentinula edodes) has been used in research involving the prevention and the control of human and plant diseases. In cucumber plants, treated with aqueous extracts from mushroom basidiocarps, there was a reduction in anthracnose severity caused by Colletotrichum lagenarium, and an increase in peroxidase activity in the leaves. With the aim of obtaining molecules of agronomic interest, the crude aqueous extract from L. edodes basidiocarp was fractioned with ammonium sulfate. The fraction corresponding to 40-80% of saturation (p40-80), the most effective in reducing anthracnose on cucumber cotyledons, was submitted to anion exchange chromatography (AEC). After AEC, six protein peaks were obtained and the peak V, containing 34% of the proteins present in p40-80, induced peroxidase increase in the cucumber cotyledons besides reducing anthracnose severity. Separation of peak V proteins by SDS-gel electrophoresis revealed the presence of more than one band in the gel. Thus, a partial purification of elicitors present in the L. edodes basidiocarp was achieved.

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