Digital monitoring of mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) on agar medium

Brazilian Journal of Microbiology

Renato Mamede De Castro Montini²  José Raimundo De Souza Passos¹  Augusto Ferreira Da Eira² 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agronômicas Departamento de Produção VegetalBotucatu SP ,Brasil

关键词: Lentinula edodes;    shiitake;    substrate;    mycelium growth;    strains;    Lentinula edodes;    shiitake;    substratos;    crescimento miceliano;    linhagens;   

DOI  :  10.1590/S1517-83822006000100017

来源: SciELO

【 摘 要 】

The mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) strains LE 96/17, LE 98/51, LE 98/53, and LE 98/56 were studied under different agar medium compositions. The strains were from the mycological collection of the Módulo de Cogumelos, Faculdade de Ciências Agronômicas, Unesp-Botucatu, Brazil. Mycelium fragments from stock cultures were transferred to Petri dishes with Sawdust extract-Dextrose-Agar medium. The area of growth and vigor (density) of the mycelia were daily recorded with a digital camera, during incubation, until the complete colonization of the Petri dish. The images were analyzed by the freeware UTHSCSA ImageTool, v. 2.0, developed by the University of Texas Health Science Center, San Antonio. The kinetics of mycelium growth, as measured by the mycelium area (mm²), has as a deterministic component an exponential function of Gompertz. The vigor, as evaluated by mycelium color in gray scale, was similar for all strains, reached a maximal value between the 4th and 5th day of incubation and decreased further on. The velocity of growth of L. edodes strains was lower in enriched culture media, while vigor was higher. Digital monitoring permits a objective evaluation of the growth kinetics of L. edodes in vitro.

【 授权许可】

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