Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children

Brazilian Journal of Microbiology

Rita De Cássia Mardegan¹  Marlise Inêz Klein¹  Magda Baglione Golvea¹  Janaina Aparecida Oliveira Rodrigues¹  Reginaldo Bruno Gonçalves¹  José Francisco Höfling¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Odontologia Departamento de Diagnóstico OralPiracicaba SP ,Brasil

关键词: Candida albicans;    genotyping;    proteinase;    phospholipase;    virulence;    Candida albicans;    genotipagem;    proteinase;    fosfolipase;    virulência;   

DOI  :  10.1590/S1517-83822006000100005

来源: SciELO

【 摘 要 】

Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no differences between the two groups. The AP-PCR method was effective in demonstrating intra-individual genetic polymorphism in C. albicans, showing a greater clonal diversity in caries-active versus caries-free children. Dendograms of similarity showed only intra-individual clonal lineage. The results suggest that the enzymatic profile does not depend on the genotypic characteristics of the strains.

【 授权许可】

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