Molecular subtyping of Campylobacter jejuni subsp. jejuni strains isolated from different animal species in the state of São Paulo, Brazil

Brazilian Journal of Microbiology

Eliana Scarcelli²  Rosa Maria Piatti²  Ricardo Harakava¹  Simone Miyashiro²  Flora Maria De Campos Fernandes¹  Fabíola Ribeiro Campos²  Waldemar Francisco¹  Margareth Élide Genovez²  Leonardo José Richtzenhain¹ 

[1] ,Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Animal São Paulo SP ,Brasil

关键词: Campylobacter jejuni;    subtyping;    sequencing;    flagellin;    Campylobacter jejuni;    subtipagem;    seqüenciamento;    flagelina;   

DOI  :  10.1590/S1517-83822005000400014

来源: SciELO

【 摘 要 】

The objective of the present trial was to characterize genetically strains of Campylobacter jejuni subsp. jejuni isolated from humans and several animal sources (bovines, swine, dogs, primates, wild boars and poultry). A total of 828 different animal samples (feces, carcass, aborted fetus and hysterectomized uterus) were analysed by means of routine bacteriological methods, and 36 C. jejuni strains were isolated. Thirty strains of human fecal origin were obtained in clinical analysis laboratories in the city of São Paulo. The 66 C. jejuni strains isolated were submitted to genetic characterization. Primers based on fla A gene were used in a polymerase chain reaction (PCR) and amplified a fragment of the 702 bp. PCR products were evaluated by means of sequencing and genealogic analysis. Genetic variability analysis of 66 strains showed 44 different subtypes of C. jejuni. One subtype was identical to a C. jejuni strain of human origin with the sequence in the GenBank (GENBANK accession number AF050186). Subtyping analysis of C. jejuni strains based on sequencing of the fla A gene variable region and analysis of sequence alignment by the Maximum Parsimony method showed to be highly discriminatory, providing the best conditions to differentiate strains involved in outbreaks from those sporadically isolated. This is the first study of molecular subtyping analysis of human and animal C. jejuni strains using sequencing technique and genealogic analysis in the state of São Paulo, Brazil.

【 授权许可】

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