Benzene, toluene and xylene biodegradation by Pseudomonas putida CCMI 852

Brazilian Journal of Microbiology

Marcelo Henrique Otenio²  Maria Teresa Lopes Da Silva¹  Maria Luiza Oliveira Marques¹  José Carlos Roseiro¹  Ederio Dino Bidoia² 

[1] ,Universidade Estadual Paulista Departamento de Bioquímica e Microbiologia Instituto de BiociênciasRio Claro SP ,Brasil

关键词: Pseudomonas putida;    biodegradation;    BTX;    plasmid TOL;    Pseudomonas putida;    biodegradação;    BTX;    plasmídeo TOL;   

DOI  :  10.1590/S1517-83822005000300010

来源: SciELO

【 摘 要 】

A minimal liquid medium containing benzene (B), toluene (T) and xylene (X) and mixtures thereof, was used to evaluate degradation activity of Pseudomonas putida CCMI 852 containing a TOL plasmid. Experiments were developed with B, T and X (100 mg L-1), with mixtures of BT, BX, and TX (50 + 50 mg L-1 each) and BTX (33.3 + 33.3 + 33.3 mg L-1 each), added to 500 mL of medium. After 18 to 24 hours, the inoculum was added and solvent disappearance was determined after 24 to 25 hours by GC. Results showed that P. putida CCMI 852 was able to metabolize T and X, but B was not metabolized. In a BTX mixture, B was not metabolized and T and X degradation rate decreased 50%.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License