Production of glucosyltransferase by Erwinia sp. using experimental design and response surface methodology

Brazilian Journal of Microbiology

Haroldo Yukio Kawaguti¹  Eiric Manrich¹  Luciana Francisco Fleuri¹  Hélia Harumi Sato¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Engenharia de Alimentos Departamento de Ciência de Alimentos

关键词: glucosyltransferase;    isomaltulose;    optimization;    response surface;    glicosiltransferase;    isomaltulose;    otimização;    superfície de resposta;   

DOI  :  10.1590/S1517-83822005000300005

来源: SciELO

【 摘 要 】

Glucosyltransferase produced by strain Erwinia sp. is an intracellular enzyme that catalyzes the formation of isomaltulose from sucrose. Isomaltulose is a non-cariogenic reducing dissacharide commercially used in foods. Response surface methodology and 2³-factorial central composite design were employed to optimize a fermentation medium for the production of glucosyltransferase by Erwinia sp. in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses (SCM), corn steep liquor (CSL) and yeast extract Prodex Lac SD (YEP). The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect on glucosyltransferase production and the optimum medium composition for enzyme production was (in g l-1) SCM-100, CSL-60 and YEP-8, which lead to a glucosyltransferase activity of 6.65 U mL-1.

【 授权许可】

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