Prevalence and molecular analysis of Anaplasma platys in dogs in Lara, Venezuela

Brazilian Journal of Microbiology

Haibin Huang²  Ahmet Unver²  Miriam J. Perez¹  Nelson G. Orellana¹  Yasuko Rikihisa² 

[1] ,The Ohio State University College of Veterinary Medicine Department of Veterinary BiosciencesColumbus Ohio ,U.S.A.

关键词: 16S rRNA;    groESL operon;    Anaplasma platys;    dogs;    Venezuela;    16S rRNA;    groESL operon;    Anaplasma platys;    cães;    Venezuela;   

DOI  :  10.1590/S1517-83822005000300002

来源: SciELO

【 摘 要 】

Blood specimens from clinically normal military dogs and their trainers, in Lara, Venezuela were screened for Anaplasma platys, A. phagocytophilum, or Ehrlichia ewingii using 16S rRNA PCR tests. Sixteen percent (7/43) of dog specimens were positive by A. platys PCR test followed by sequencing of the PCR products, and all human blood specimens [25] were negative. All specimens from these dogs and humans were PCR negative for E. ewingii or A. phagocytophilum. Twelve Rhipicephalus sanguineus ticks removed from these dogs were negative for A. platys by reverse transcription PCR test. Almost the entire 16S rRNA gene (1,364 bp) and groESL operon (1,646 bp) sequences of A. platys isolated from a dog were determined, revealing that both sequences were closely related to the sequences of an A. platys strain detected in R. sanguineus ticks from the Democratic Republic of Congo.

【 授权许可】

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