Detection of Leptospira spp. in the semen and urine of bulls serologically reactive to Leptospira interrogans serovar hardjo

Brazilian Journal of Microbiology

Fernanda Senter Magajevski²  Raul Jose Silva Girio²  Luis Antonio Mathias²  Simone Myashiro¹  Margareth Élide Genovez¹  Eliana Pinheiro Scarcelli¹ 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Medicina Veterinária PreventivaJaboticabal SP ,Brasil

关键词: Leptospira;    semen;    urine;    PCR;    Leptospira;    sêmen;    urina;    PCR;   

DOI  :  10.1590/S1517-83822005000100009

来源: SciELO

【 摘 要 】

This study evaluated PCR for the detection of leptospires in semen and urine of ten serologically reactive bulls, comparing these results with those obtained with other diagnosis techniques. Two collections of materials were done in alternate days. Semen and urine samples were separated in aliquots for: direct visualization in dark field microscopy; inoculation in hamsters (for semen only); isolation in culture media; and PCR. No hamster was positive by the microscopic agglutination test (MAT); kidney and liver fragments from the hamsters were used in an isolation attempt in culture media, with one positive isolation from the kidney of a hamster inoculated with semen of one bull, and from liver of hamsters inoculated with semen of three bulls. Isolation in culture was negative for all semen samples, but positive for five urine samples by direct inoculation. In PCR there was no positive result for semen samples, and only one urine sample was positive, which was coincident with one of the positive cultures. It was not possible to visualize leptospires in any of the samples by dark field microscopy.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License