Production of hydrolytic enzymes by the plant pathogenic fungus Myrothecium verrucaria in submerged cultures

Brazilian Journal of Microbiology

Fabiana Guillen Moreira¹  Simone Dos Reis¹  Maria Aparecida Ferreira Costa¹  Cristina Giatti Marques De Souza¹  Rosane Marina Peralta¹ 

[1] ,Universidade Estadual de Maringá Departamento de Bioquímica Maringá PR ,Brasil

关键词: carbohydrases;    hydrolytic enzymes;    Myrothecium verrucaria;    protease;    carboidrases;    enzimas hidrolíticas;    Myrothecium verrucaria;    protease;   

DOI  :  10.1590/S1517-83822005000100002

来源: SciELO

【 摘 要 】

The capability of the plant pathogenic fungus Myrothecium verrucaria to produce extracellular hydrolytic enzymes in submerged cultures was studied using several substrates. The fungus was able to produce different depolymerases and glycosidases, being xylanase, pectinase and protease the most important. Lipase was found in cultures developed in the presence of olive oil, while protease activity was detected in all cultures. Xylanase and pectinase were optimally active at pH 4.5-5.5, while protease was active in a large range of pH 3.5 to 11.0. All three enzymes were maximally active at 40ºC and they were stable for several hours at temperature up to 50ºC.

【 授权许可】

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