Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry

Brazilian Journal of Microbiology

Telma Luisa De Freitas²  Rubens Monti¹  Jonas Contiero¹ 

[1] ,Universidade Estadual Paulista Júlio Mesquita Filho Departamento de Bioquímica e Tecnologia Araraquara SP ,Brasil

关键词: Bacillus alkalophilic CGII;    CGTase;    beta-cyclodextrin;    starch hydrolysis;    enzyme activity;    Bacillus alkalophilic CGII;    CGTase;    beta-ciclodextrina;    hidrólise do amido;    atividade enzimática;   

DOI  :  10.1590/S1517-83822004000200015

来源: SciELO

【 摘 要 】

GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License