Fate of Helicobacter pylori artificially inoculated in lettuce and carrot samples

Brazilian Journal of Microbiology

Bruna C. Gomes¹  Elaine C.p. De Martinis¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Análises Clínicas, Toxicológicas e BromatológicasRibeirão Preto SP ,Brasil

关键词: H. pylori;    peptic ulcer;    modified atmosphere;    vegetable;    H. pylori;    úlcera péptica;    atmosfera modificada;    vegetais;   

DOI  :  10.1590/S1517-83822004000100024

来源: SciELO

【 摘 要 】

Helicobacter pylori is known, worldwide, as the causative agent of gastric diseases. However, its transmission route has not been completely understood. To evaluate the survival of H. pylori (a clinical and a reference strain) artificially inoculated on lettuce and carrot samples, portions of 25 g were inoculated with approximately 10(6) CFU/g of H. pylori and packed under normal and/or modified atmosphere (3% oxygen, 10% carbon dioxide, and 87% nitrogen). The inoculated food samples were stored at 8ºC, with daily enumeration of H. pylori populations on Columbia blood agar (CBA) and/ or Helicobacter pylori Special Peptone Agar (HPSPA). When CBA with antibiotics was used, the clinical isolate H. pylori HP1 was detected for up to 72 h in sanitized lettuce and carrot. In sterilized carrot samples, H. pylori HP1 remained viable for up to 96 h. The CBA without antibiotics allowed the recovery of H. pylori ATCC 43629, from carrot samples stored in both atmospheres tested, for up to 120 hours. Our results reinforce that foodborne transmission of H. pylori cannot be disregarded yet.

【 授权许可】

CC BY-NC   
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