Effect of the Kozak sequence on seroconversion of mice immunized with a DNA vaccine against swine colibacilosis

Brazilian Journal of Microbiology

André Michelon²  Marcelo Michelon²  Simone Simionatto²  Valeska Lizzi Lagranha²  Fabrício Rochedo Conceição²  Eliana Knackfus Vaz¹  Gustavo Maia Cerqueira²  Odir Antonio Dellagostin² 

[1] ,Universidade Federal de Pelotas Campus Universitário Capão do Leão Centro de BiotecnologiaPelotas RS ,Brasil

关键词: swine colibacilosis;    DNA vaccine;    faeC;    Kozak sequence;    colibacilose suína;    vacina de DNA;    faeC;    seqüência de Kozak;   

DOI  :  10.1590/S1517-83822003000500029

来源: SciELO

【 摘 要 】

The neonatal diarrhea in swine caused by enterotoxigenic Escherichia coli (ETEC) is responsible for high mortality and low growth rate in pigs and it is mainly dependent on the capacity of E. coli to attach to the surface of the small intestine, a property mediated by fimbria. In this study the faeC gene, which codes for the minor fimbrial subunit of E. coli K88ab, was cloned in the eukaryotic expression vector pcDNA3, associated or not to the Kozak sequence. Plasmid DNA of the two versions of the vaccine candidate was inoculated in mice by the intramuscular route, in two doses, at 0 and 21 days. The animals that received the DNA vaccine containing faeC associated to the Kozak sequence presented seroconversion significantly higher (P<0.05) than the one vaccinated with pcDNA3/faeC without the Kozak sequence.

【 授权许可】

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