Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires

Brazilian Journal of Microbiology

Núbia Seyffert¹  Éverton Fagonde Da Silva¹  Viviane Lorenzato Bermudez¹  Claudiomar Soares Brod¹ 

[1] ,Universidade Federal de Pelotas Faculdade de Medicina Veterinária Centro de Controle de ZoonozesPelotas RS ,Brasil

关键词: Leptospira;    microscopic agglutination;    Leptospirosis;    laboratorial diagnosis;    Leptospira;    soroaglutinação microscópica;    Leptospirose;    diagnóstico laboratorial;   

DOI  :  10.1590/S1517-83822003000500025

来源: SciELO

【 摘 要 】

In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.

【 授权许可】

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