Use of molecular approach to verify the influence of a eutrophic lagoon in the nearby ocean's bacterioplankton communities

Brazilian Journal of Microbiology

Rodrigo Gouvêa Taketani²  Fábio Vieira Araujo¹  Margaretha Denise Maria Van Weerelt¹  Maria Leonisa Sanchez-nunez¹  Erick Aniszewski²  Alexandre Soares Rosado² 

[1] ,Universidade Federal do Rio de Janeiro Instituto de Microbiologia Laboratório de Ecologia Microbiana MolecularRio de Janeiro RJ ,Brasil

关键词: DGGE;    diversity;    bacterioplankton;    eutrophic lagoon;    DGGE;    diversidade;    bacterioplancton;    lagoa eutrófica;   

DOI  :  10.1590/S1517-83822003000500017

来源: SciELO

【 摘 要 】

Rodrigo de Freitas lagoon is an eutrophic aquatic environment. The waters from the lagoon are released to the sea at Ipanema and Leblon beaches, through Jardim de Alah channel. In this work, the influence of these waters on the bacterial communities of these beaches was investigated. Eleven sampling stations were set between the lagoon and the beaches, and the samples were analyzed by molecular and microbiological parameters. PCR-DGGE of the DNA extracted from the samples was performed using rpoB primers. Preliminary results indicate that all used approaches could reveal the influence of the lagoon on the beaches bacterial communities.

【 授权许可】

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