Determination of decimal reduction time (D-value) of chemical agents used in hospital disinfection

Brazilian Journal of Microbiology

Priscila Gava Mazzola¹  Alzira Maria Da Silva Martins¹  Thereza Christina Vessoni Penna¹ 

[1] ,Universidade de São Paulo Departamento de Tecnologia Bioquímico Farmacêutica São Paulo,Brasil

关键词: decimal reduction time;    sanitizers;    desinfection;    tempo de redução decimal;    sanitizantes;    desinfecção;   

DOI  :  10.1590/S1517-83822003000500011

来源: SciELO

【 摘 要 】

Prior to selecting disinfectant for low, intermediate and high (sterilizing) levels, the decimal reduction time, D-value, for the most common or persistent bacteria identified on a medical device or at a health care facility should be determined. The D-value was determined by inoculating 100 mL of disinfecting solution with 1 mL of a bacterial suspension. At regular intervals, 1 mL aliquots of this mixture were transferred to 8 mL of growth media containing a neutralizing agent, and incubated at optimal conditions for the microorganism. B. stearothermophilus and E. coli were the most resistant bacteria for the disinfecting and sterilizing procedures.

【 授权许可】

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