Behavior of Leishmania major metacyclic promastigotes during the course of infection and immune response development in resistant versus susceptible hosts

Brazilian Journal of Microbiology

Regina Coeli Cunha Dórea²  Carlos Gomes Barboza-filho²  Dayson Friaça Moreira²  Mirian Guirro²  Ágtha De Alencar Muniz Chaves²  Ricardo José Dunder²  Elaine Amorim Soares¹  Vitório Santos-júnior¹  Wilma Spinosa¹ 

[1] ,Universidade Estadual Paulista Campus de Assis Faculdade de Ciências e LetrasAssis SP ,Brasil

关键词: Leishmania major;    metacyclic promastigotes;    metacyclogenesis;    BALB/c mice;    C57BL/6 mice;    Leishmania major;    promastigotas metacíclicos;    metaciclogênese;    camundongos BALB/c;    camundongos C57BL/6;   

DOI  :  10.1590/S1517-83822003000500006

来源: SciELO

【 摘 要 】

Little is known on the epitopes derived from metacyclic promastigotes of Leishmania that are important on the regulation or destruction of the parasite, as targets of immune attack in the vertebrate host. In this study we investigated an alternative method to obtain metacyclic promasigotes of Leishmania major, as evaluated by the course of infection and delayed-type hipersensitivity (DTH) in resistant versus susceptible inbred mice. Non-infective (procyclic) promastigotes of L. major recently transformed from tissue amastigotes were attached to a negatively charged glass-wool column, whereas metacyclic promastigotes were not bound to columns and could be easily recovered. Optimal chromatography conditions were validated through statistical analyses. Parasite average yield from glass wool columns and promastigote viability were estimated by light microscopy. Metacyclic promastigotes yielded 43.5% to 57.5%. Different patterns of cutaneous lesions were obtained in BALB/c (susceptible) and C57BL/6 (resistant) mice, the former with highly infective lesions induced by metacyclic promastigotes. DTH responses proved to be higher in groups of C57BL/6 mice which were infected with metacyclic promastigotes. These results indicate that the new method could be integrated with the investigation of metacyclogenesis of Leishmania in vivo.

【 授权许可】

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