Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains

Brazilian Journal of Microbiology

Priscila N. Ferraz¹  Maria Das Graças M. Danelli¹ 

[1] ,Universidade Federal Rural do Rio de Janeiro Instituto de Veterinária Departamento de Microbiologia e Imunologia Veterinária

关键词: Mycoplasma gallisepticum;    phenotypic variation;    antigenic variation;    Mycoplasma gallisepticum;    variação fenotípica;    variação antigênica;   

DOI  :  10.1590/S1517-83822003000300011

来源: SciELO

【 摘 要 】

Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine F and Ts-11 strains were investigated by SDS-PAGE and serological methods (hemagglutination-inhibition and immunoblot assays). The SDS-PAGE system followed by densitometer analysis showed weak phenotypic variability between the strains, being the major difference close to the 75 kDa level where a prominent peptide band was detected only in the F vaccine strain. Polyclonal antibodies to the M. gallisepticum antigens produced in chickens were used in serological tests for this antigenic variability study. There were strong cross-reactions between the strains and homologous/ heterologous antibodies. The most evident characteristic was the specific response of the vaccine-type F polyclonal antiserum to the 75 kDa peptide band of the homologous strain.

【 授权许可】

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