Dominant culturable bacterial microbiota in the digestive tract of the American black vulture (Coragyps atratus Bechstein 1793) and search for antagonistic substances

Brazilian Journal of Microbiology

Lydston Rodrigues De Carvalho¹  Luiz Macêdo Farias¹  Jacques Robert Nicoli¹  Maria Clara Fernandes Silva¹  Andrea Teresa Santos Meira Corsino¹  Luiz André De Lima¹  Rodrigo Aparecido Fernandes Redondo¹  Paulo César Peregrino Ferreira¹  Maria Elizabeth Bernardes Margutti Pinto¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de MicrobiologiaBelo Horizonte Minas Gerais ,Brasil

关键词: Culture;    microbiota;    digestive tract;    antagonistic substance;    urubu;    microbiota;    trato digestivo;    substância antagonista;   

DOI  :  10.1590/S1517-83822003000300007

来源: SciELO

【 摘 要 】

Strict and facultative culturable anaerobic bacteria from the digestive tract of six American black vultures (Coragyps atratus Bechstein 1793) were isolated and identified. After capture, the birds received a non-contaminated diet for one week to eliminate possible allochthonous microorganisms. Then, specimens collected from tongue, stomach and intestines were weighed, submitted to decimal dilution in an anaerobic chamber, inoculated into culture media and incubated aerobically and anaerobically at 37ºC for enumeration, isolation and identification. Isolated bacteria were submitted to tests to detect possible antagonisms between them. The total bacterial population along the digestive tract ranged from 3.46 ± 0.39 log CFU/g in the stomach to 10.75 ± 0.37 log CFU/g in the distal intestine. Some bacteria were isolated for the first time from the digestive tract of C. atratus: Actinomyces bovis, Lactobacillus cellobiosus, Micrococcus luteus, Neisseria sicca, Clostridium bifermentans, Enterobacter agglomerans, Peptostreptococcus sp., Sarcina sp., Serratia odorifera, and Shigella flexneri. Associations between microorganisms were observed during isolation on two occasions, one involving A. bovis and N. sicca, and the other involving A. bovis and a Gram-negative rod. Hetero-, iso- and autoantagonisms were observed, suggesting the ecological role of these indigenous microorganisms in terms of population auto-control and environmental barrier in the digestive tract of carrion-feeding birds.

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