Detection of rotavirus in dogs with diarrhea in Brazil

Brazilian Journal of Microbiology

Yvone B. Gabbay²  Valéria S.f. Homem¹  Veridiana Munford¹  Antonia S. Alves²  Joana D.p. Mascarenhas²  Alexandre C. Linhares²  Maria Lúcia Rácz¹ 

[1] ,Fundação Nacional de Saúde Instituto Evandro Chagas Seção de Virologia Geral

关键词: rotavirus;    diarrheic dog;    genotype G;    genotype P;    Belém- Brazil;    rotavirus;    diarréia canina;    genotipo G;    genotipo P;    Belém-Brasil;   

DOI  :  10.1590/S1517-83822003000100016

来源: SciELO

【 摘 要 】

Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P[ 3] genotype. This is the first canine rotavirus detected in Brazil.

【 授权许可】

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