Production of hydrolytic enzymes by Trichoderma isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches' broom of cocoa

Brazilian Journal of Microbiology

Janice Lisboa De Marco²  Maria Cléria Valadares-inglis¹  Carlos Roberto Felix² 

[1] ,Universidade de Brasília Departamento de Biologia Celular Brasília DF ,Brasil

关键词: Trichoderma;    Crinipellis perniciosa;    hydrolases;    antagonistic interaction;    Trichoderma;    Crinipellis perniciosa;    hidrolases;    interação antagonística;   

DOI  :  10.1590/S1517-83822003000100008

来源: SciELO

【 摘 要 】

Two isolates of Trichoderma, which reduce the incidence of witches'broom disease caused in cocoa by Crinipellis perniciosa, were evaluated for their potential to produce hydrolases in liquid medium. Very low or no hydrolytic activity was produced in the absence of any substrate. The activities of chitinase, N-acetylglucosaminidase, beta-1,3-glucanase, total cellulase, endoglucanase, aryl- beta-glucosidase, beta-glucosidase, protease and amylase increased dramatically within 72-120 h of growth in the presence of specific substrates. Except for N-acetylglucosaminidase and beta-glucosidase Trichoderma harzianum isolate 1051 produced the largest amounts of hydrolases. The possible involvement of these enzymes in the antagonistic interaction between Trichoderma and C. perniciosa is discussed.

【 授权许可】

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