Doxorubicin and etoposide induce somatic recombination in diploid cells of Aspergillus nidulans

Brazilian Journal of Microbiology

Simone Jurema Ruggeri Chiuchetta¹  Marialba Avezum Alves De Castro-prado¹ 

[1] ,Universidade Estadual de Maringá Departamento de Biologia Celular e Genética Maringá Paraná ,Brasil

关键词: antineoplastic agents;    mitotic crossing-over;    secondary malignances;    agentes antineoplásicos;    crossing-over mitótico;    malignidade secundária;   

DOI  :  10.1590/S1517-83822002000300014

来源: SciELO

【 摘 要 】

Doxorubicin and etoposide are intercalating agents that inhibit the action of the enzyme topoisomerase II. Both drugs present therapeutic activity in numerous human neoplasms In the present work the recombinagenic potential of these drugs was evaluated by ascomycete Aspergillus nidulans. Their effects on the asexual cycle of A. nidulans was also appraised. Two heterozygous diploid strains of A. nidulans, a wild (uvsH+//uvsH+) and a defective to the DNA repair (uvsH//uvsH) were used. The drugs' recombinagenic potential was evaluated by their capacity to induce homozygosis of recessive genes from heterozygous cells. Both drugs have a recombinagenic effect on diploid cells of A. nidulans. Doxorubicin and etoposide are potentially capable to induce secondary malignancies, mediated by the mitotic crossing-over in eukaryotic cells.

【 授权许可】

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