Trehalose accumulation, invertase activity and physiological characteristics of yeasts isolated from 24 h fermentative cycles during the production of artisanal Brazilian cachaça

Brazilian Journal of Microbiology

Carla Pataro²  Juliana B. Guerra¹  Fátima C.o. Gomes¹  Maria J. Neves¹  Patrícia F. Pimentel²  Carlos A. Rosa¹ 

[1] ,Fundação Centro Tecnológico de Minas Gerais Setor de Biotecnologia e Tecnologia Química Belo Horizonte MG ,Brazil

关键词: Saccharomyces cerevisiae;    cachaça;    trehalose;    invertase;    fermentation;    Saccharomyces cerevisiae;    cachaça;    trealose;    invertase;    fermentação;   

DOI  :  10.1590/S1517-83822002000300003

来源: SciELO

【 摘 要 】

Trehalose accumulation, invertase activity and physiological characteristics of 86 yeast isolates from short fermentative cycles during the production of cachaça in three artisanal distilleries of the State of Minas Gerais were studied. Among these isolates, 70% were able to grow at temperatures between 40 and 42ºC. Only Saccharomyces cerevisiae isolates were able to grow over 40ºC. Lower temperatures (<40ºC) favoured the growth of yeasts such as Candida parapsilosis-like, C. maltosa-like, Kloeckera japonica, S. exiguus and C. bombicola-like. The isolates from all three distilleries were ethanol tolerant, produced invertase, and accumulate trehalose in the presence of glucose. The strains isolated from distillery A presented more resistance to ethanol (around 84.2% of the strains were able to grow in the presence of 12% ethanol) when compared to the ones from distilleries C and B (9.5% and no strain, respectively). The strains of S. cerevisiae isolated from the three distilleries presented a higher capacity to produce invertase and accumulate trehalose in the presence of glucose. Based on the results of thermal and ethanol stress experiments, it was possible to identify strong relationship between intracellular trehalose accumulation and cell viability. The increase in cell viability was even more pronounced when the strains were subjected to a pre-treatment at sublethal temperatures.

【 授权许可】

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