Production of extracellular proteases by Mucor circinelloides using D-glucose as carbon source / substrate

Brazilian Journal of Microbiology

Vânia Sousa Andrade²  Leonie Asfora Sarubbo¹  Kasutaka Fukushima¹  Makoto Miyaji¹  Kazuko Nishimura¹  Galba Maria De Campos-takaki¹ 

[1] ,Universidade Federal de Alagoas Departamento de Patologia Maceió AL ,Brasil

关键词: extracellular proteases;    D-glucose;    substrate;    Mucor circinelloides;    proteases extracelulares;    D-glicose;    substrato;    Mucor circinelloides;   

DOI  :  10.1590/S1517-83822002000200002

来源: SciELO

【 摘 要 】

Recently, some Mucorales species have been reported as protease producers. The production of extracellular proteases by Mucor circinelloides using glucose as substrate was studied. Experiments were carried out with different D-glucose concentrations (40, 60 and 80 g/L). Biomass, pH and protease activity were determined. Although biomass production had reached best yields for the medium containing D-glucose in a concentration of 80 g/L, the enzymatic production was higher when the substrate concentration was reduced to 40 g/L. The yield factor for product on cell growth and the yield factor for product on carbon substrate were higher when the microorganism grew in medium containing 40 g/L glucose. The kinetics parameters suggest that this strain seems to be promising as an alternative microorganism for protease production.

【 授权许可】

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