The interactive effect of phosphorus and nitrogen on "in vitro" spore germination of Glomus etunicatum Becker & Gerdemann, root growth and mycorrhizal colonization

Brazilian Journal of Microbiology

Wellington Bressan¹ 

[1],Embrapa Milho e Sorgo Departamento de Microbiologia Sete Lagoas MG ,Brasil

关键词: Mycorrhiza;    Glomus etunicatum;    spore germination;    root growth;    root colonization;    Micorriza;    Glomus etunicatum;    germinação de esporos;    colonização de raízes;    crescimento de raízes;   

DOI  :  10.1590/S1517-83822001000400004

来源: SciELO

【 摘 要 】

The effects of P and N amendment and its interactions on spore germination, root growth and colonized root length by Glomus etunicatum Becker & Gerdemann (INVAM S329) was studied "in vitro" in RiT - DNA transformed roots of Anthylis vulneraria sub sp. Sampaiana (Kidney vetch). Three N media concentrations (5, 10 and 50 mg/l) at P constant level (2 mg/l) and three P media concentrations (2, 10 and 20 mg/l) at N constant level (5 mg/l) were utilized as a treatment. Bécard & Fortin medium was used as a basal medium for root growth and colonized root length, and water/agar (0.8%) media was the control for spore germination. Spore germination of G. etunicatum at low P level was reduced by N addition in relation to the control media, and at low N level addition of P stimulated spore germination. Total root length was stimulated by N addtion at low P level, but no significant difference (p£0.05) was observed between 10 and 50 mg/l of N. P addition at low N level media also stimulated total root growth, and a significant difference (p£0.05) was observed among P concentrations. Colonized root length by G. etunicatum increased significantly (p£0.05) with P additions at low N levels. Under low P level no significant differences was found between 10 and 50 mg/l of N. These results demonstrate that the interaction between P and N affect differently spore germination, root growth and colonized root lenght.

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