Brazilian Journal of Microbiology | |
EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO | |
Maria De Fátima Costa Pires2  Benedito Corrêa1  Walderez Gambale1  Claudete Rodrigues Paula1  | |
[1] ,Instituto Adolfo Lutz Secão de Microscopia Eletrônica São Paulo SP ,Brasil | |
关键词: Candida albicans; adherence; serotypes; Candida albicans; aderência; sorotipos; | |
DOI : 10.1590/S1517-83822001000300001 | |
来源: SciELO | |
【 摘 要 】
The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
【 授权许可】
CC BY-NC
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