A method for the determination of two Alternaria toxins, alternariol and alternariol monomethyl ether, in tomato products

Brazilian Journal of Microbiology

Silvana Da Motta¹  Lucia M. Valente Soares¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Engenharia de Alimentos Campinas SP ,Brasil

关键词: Alternaria;    mycotoxins;    alternariol monomethyl ether;    alternariol;    tomatoes;    Alternaria;    micotoxinas;    alternariol monometil éter;    alternariol;    tomate;   

DOI  :  10.1590/S1517-83822000000400015

来源: SciELO

【 摘 要 】

Tomatoes, as any soft skinned fruit, are easily susceptible to fungal rot. Alternaria is the genus most frequently encountered in tomatoes. A. alternata and other spp. have been shown to produce the toxins alternariol monomethyl ether (AME) and alternariol (AOH) in tomatoes. A method for determining AME and AOH in tomato products was developed and evaluated. The method involves extraction with methanol, clarification with ammonium sulfate, and partition to chloroform. Quantification was conducted by high performance liquid chromatography with diode array detector (DAD). Average recoveries were 98.7% and 84.1% for AME and AOH, respectively. The quantification limits of the method, defined as the minimum amount that allowed quantification and confirmation by the DAD detector, were 2.0 ng/g for AME and 5.0 ng/g for AOH.

【 授权许可】

CC BY-NC   
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