Protein profile analysis by SDS-PAGE of Mycoplasma Gallisepticum strains S6(208) and F-K810 grown in Hayflick's and Frey's media

Brazilian Journal of Microbiology

Priscila N. Ferraz²  Claudio C. P. Menezes²  Maria Das Graças M. Danelli²  Jackeline De O. P. Lizeu²  Elmiro R. Do Nascimento¹  Daniela M. Lucchesi¹ 

[1] ,Universidade Federal Rural do Rio de Janeiro Instituto de Veterinária Seropédica RJ ,Brasil

关键词: Mycoplasma gallisepticum;    SDS-PAGE;    protein profile;    Mycoplasma gallisepticum;    SDS-PAGE;    perfil protéico;   

DOI  :  10.1590/S1517-83822000000200010

来源: SciELO

【 摘 要 】

The Mycoplasma gallisepticum strains [wild-type S6 (208) and a vaccine-type F-K810] grown in Frey´s and Hayflick´s media were analyzed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.

【 授权许可】

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