Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola

Brazilian Journal of Microbiology

Jacqueline Aparecida Takahashi²  Henrique De Almeida Barroso¹  Alaíde Braga De Oliveira¹ 

[1],Universidade Federal de Minas Gerais ICEx Departamento de QuímicaBelo Horizonte MG ,Brasil

关键词: Cephalosporium aphidicola;    biotransformation;    diterpene;    Cephalosporium aphidicola;    biotransformação;    diterpenos;   

DOI  :  10.1590/S1517-83822000000200003

来源: SciELO

【 摘 要 】

Parameters for a more efficient biotransformation of diterpene-like compounds by the fungus Cephalosporium aphidicola were established by carrying out microscale feedings at several conditions. Experiments were guided by thin layer chromatography and gas chromatography analysis. It was observed that the substrate should be added in ethanol at concentrations between 15 to 30 mg per 100 ml of medium. The extraction of the product showed to be more efficient when carried out from both mycelia and broth and using ethylacetate as the extracting solvent. The experiment should be stopped six days after feeding the substrate to the fungus for the best product yield.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License