A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium

Brazilian Journal of Microbiology

Aline E. Nascimento²  Allana E. Do Nascimento Shariá¹  Marco Antonio Barbosa De Lima¹  Galba Maria Campos-takaki² 

[1] ,Universidade Católica de Pernambuco Núcleo de Pesquisas em Ciências Ambientais Departamentos de Biologia e QuímicaRecife PE ,Brasil

关键词: Candida lipolytica;    carbohydrate cytochemistry;    fungi ultrastructure;    Candida lipolytica;    citoquímica de carboidratos;    ultraestrutura de fungos;   

DOI  :  10.1590/S1517-83822000000100008

来源: SciELO

【 摘 要 】

Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.

【 授权许可】

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