Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic

Brazilian Archives of Biology and Technology

Eleonidas Moura Lima¹  Otávio Sérgio Lopes¹  Leonardo Ferreira Soares¹  Talitta Dantas Arruda¹  Carolina Oliveira Gigek¹  Cynthia Germoglio Farias Melo¹  Marilia Arruda Cardoso Smith¹  João Ricardo Gonçalves Oliveira¹  Arnaldo Medeiros¹  Plínio Delatorre¹  Rommel Rodriguez Burbano¹ 

关键词: DSASP method;    genotyping;    Gastric Cancer;   

DOI  :  10.1590/S1516-8913201500434

来源: SciELO

【 摘 要 】

Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.

【 授权许可】

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