The investigation of cytogenetic and oxidative effects of diffractaic acid on human lymphocyte cultures

Brazilian Archives of Biology and Technology

Leyla Demir¹  Başak Toğar¹  Hasan Türkez¹  Piera Sozio¹  Ali Aslan¹  Antonio Di Stefano¹ 

关键词: Diffractaic acid;    cytotoxicity;    human blood;    genotoxicity;    oxidative status;   

DOI  :  10.1590/S1516-8913201502752

来源: SciELO

【 摘 要 】

Diffractaic acid (DA) is a naturally occurring depside derivative found in several lichen species. It has a wide range of important biological effects such as analgesic and antiviral properties, although its cytotoxic, cytogenetic and oxidative effects have not been investigated in human blood tissue yet. Therefore, increasing concentrations (1, 5, 10, 25, 50, 100 and 200 mgL-1) of DA was added into human whole blood cultures. 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay was used to assess the cell viability and/or cytotoxicity and genotoxic damage potential of DA using chromosome aberration (CA) and micronucleus (MN) tests were performed. In addition, oxidative alterations were determined by the total antioxidant capacity (TAC) and total oxidant status (TOS) assays. The results revealed that DA reduced cell viability at higher concentrations than 50 mgL-1. The all tested concentrations of DA were non-genotoxic. In vitro treatments with DA led to increases of TAC levels in the cultured blood cells without changing the TOS levels as compared to the control group. Consequently, DA exhibited a significant non-mutagenic and antioxidant potential in vitro.

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