Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit

Brazilian Archives of Biology and Technology

Milene Alves De Figueiredo Carvalho¹  Renato Paiva¹  Vanessa Cristina Stein¹  Raírys Cravo Herrera¹  Jorge Marcelo Padovani Porto¹  Daiane Peixoto Vargas¹  Eduardo Alves¹ 

关键词: Passifloraceae;    callogenesis;    electromicroscopy;    optic microscope;    tissue culture;   

DOI  :  10.1590/S1516-8913201402555

来源: SciELO

【 摘 要 】

This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli.

【 授权许可】

CC BY-NC   
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