Enhanced Production of Vitamin K2 from Bacillus subtilis (natto)by Mutation and Optimization of the Fermentation Medium

Brazilian Archives of Biology and Technology

Junying Song¹  Hongxia Liu¹  Li Wang¹  Jun Dai¹  Yan Liu¹  Hui Liu¹  Genhai Zhao¹  Peng Wang¹  Zhiming Zheng¹ 

关键词: Bacillus subtilis (natto);    Mutant;    Optimization;    Vitamin K2;   

DOI  :  10.1590/S1516-8913201402126

来源: SciELO

【 摘 要 】

The aim of this study was to enhance the production of vitamin K2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG) and low energy ion beam implantation and optimizing the fermentation medium. Mutation resulted in 1.66-fold higher production of vitamin K2 than that of the parentl strain. The production by the mutant BN-P15-11-1was increased 55% and reached 3.593±0.107 mg/L by using the Plackett-Burman and Box-Behnken designs to optimize the fermentation medium. The optimal fermentation culture medium was composed of (g/L) glycerol 69.6, sucrose 34.5, K2HPO4 4.0, peptone 20, yeast extract 25 and fermented at 37 °C and 150 rpm for 72 h. The results showed that the NTG and low energy ion beam implantation mutations and optimizing fermentation medium were effective methods to enhance vitamin K2production.

【 授权许可】

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