Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles

Brazilian Archives of Biology and Technology

Lara Aparecida Buffoni Campos Carneiro¹  Tales Alexandre Costa-silva¹  Cláudia Regina Fernandes Souza¹  Luciano Bachmann¹  Wanderley Pereira Oliveira¹  Suraia Said¹ 

关键词: enzyme immobilization;    chitosan;    Cercospora kikuchii;    lipase;   

DOI  :  10.1590/S1516-8913201402174

来源: SciELO

【 摘 要 】

This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE). The immobilized enzyme retained an activity of about 53.5 % after five reuses, using p-NPP as substrate. Physical adsorption of lipase onto highly deacetylated CHM showed 46.2 % of enzymatic activity and 28.6% of IE. This immobilized derivative did not lose activity up to 80 days of storage at 4°C, while lipases immobilized on pre-activated CAM maintained its activity up to 180 days at same conditions. Taken together the results indicate that chitosan microparticles provide an optimal microenvironment for the immobilized enzyme to maintain good activity and stability.

【 授权许可】

CC BY-NC   
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