Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol

Brazilian Archives of Biology and Technology

Pedro De Oliva Neto²  Fabíola Aliaga De Lima²  Ketrin Cristina Da Silva²  Douglas Fernandes Da Silva²  Ana Flavia Azevedo Carvalho¹  Catarina Dos Santos² 

[1] ,Universidade Estadual Paulista Laboratório de Biotecnologia Industrial Departamento de Ciências BiológicasAssis SP ,Brasil

关键词: Ethanol;    lactic acid bacteria;    Saccharomyces cerevisiae;    Lactobacillus fermentum;   

DOI  :  10.1590/S1516-8913201401214

来源: SciELO

【 摘 要 】

The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best results were: 3,4,4' trichlorocarbanilide (TCC), tested at pH 4.0 (MIC = 3.12 mg/l), TCC with benzethonium chloride (CBe) at pH 6.0 (MIC = 3.12 mg/l) and TCC mixed with benzalkonium chloride (CBa) at pH 6.0 (MIC = 1.53 mg /l). If CBa was used in sugar cane milling in 1:1 ratio with TCC, a 8 times reduction of CBa was possible. This formulation also should be tested in fermentation steps since it was more difficult for the bacterium to develop resistance to biocide. There was no inhibition of S. cerevisiae and there were only antibiotics as an option to bacterial control of fuel ethanol fermentation by S. cerevisiae.

【 授权许可】

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