Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability

Brazilian Archives of Biology and Technology

Mohd Iswadi Ismail²  Khairul Osman¹  Siti Fatimah Ibrahim²  Farah Hanan Fatihah Jaafar²  Nur Azianie Abd Ghani²  Fazly Ann Zainalabidin¹  Abas Mazni Othman¹ 

[1] ,The National University of Malaysia Department of Physiology Kuala Lumpur,Malaysia

关键词: Cryopreservation;    sperm;    canister;    motility;    viability;   

DOI  :  10.1590/S1516-89132014000200006

来源: SciELO

【 摘 要 】

The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm²) and single tubing-type canister (4π x 90 mm²). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p<0.05). Significant changes were found in sperm kinetics (VCL, VAP, VSL) of tubing-type canister compared to commercial canister. No significant changes in the motility and viability of the bull sperm at the top of tubing-type canister and commercial canister. There were no significant changes in sperm progression (LIN, WOB, PROG) in both the canisters. Developed tubing-type canister in this study showed potential as an alternative to be used in bull sperm cryo-storage.

【 授权许可】

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