Evaluation of different adjuvants formulations for bluetongue vaccine

Brazilian Archives of Biology and Technology

Ludmila Branco Macedo²  Zélia Inês Portela Lobato¹  Sílvia Ligório Fialho¹  Aline De Marco Viott¹  Roberto Maurício Carvalho Guedes¹  Armando Silva-cunha² 

[1] ,Universidade Federal de Minas Gerais Faculdade de Farmacia Belo Horizonte MG ,Brasil

关键词: Emulsion;    microemulsion;    vaccine adjuvants;    immunology;    biocompatibility;   

DOI  :  10.1590/S1516-89132013005000002

来源: SciELO

【 摘 要 】

This study investigated the adjuvant potential of W/O/W multiple emulsions and microemulsions, comparing them with traditional aluminum hydroxide and oil-in-water emulsion adjuvants against bluetongue vaccine (BTV). Local inflammatory reactions were assessed in rabbits by measuring the temperature of the animals and the skin thickness at the site of application. Antibodies titers were determined by serum-neutralization test. Histological analyses of lesions at the site of adjuvants application were done. Results showed that multiple emulsion and microemulsion maintained their stability even in the presence of complex components and presented adequate characteristics for subcutaneous administration. They were able to induce immune response against BTV, but it was smaller than the traditional adjuvants. Despite microemulsion adjuvant showed lower antibodies titre, it was easier to prepare more stable at 4°C and it was the only one that did not induce any local reaction.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License