Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition

Brazilian Archives of Biology and Technology

Abdul Basit Khan²  Mehmood Iqbal Khattak¹  Omer Mukhtar Tarar²  Farzana Habib¹  Khalid Jamil²  Arfa Yasmin¹  Shoukat Parvez¹ 

[1] ,Food and Marine Resources Research Centre PCSIR Laboratories Complex Karachi,Pakistan

关键词: Polyhydroxyalkanoates;    Biodegradable polymers;    PHA synthase gene;    FTIR;   

DOI  :  10.1590/S1516-89132013000400015

来源: SciELO

【 摘 要 】

In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR.

【 授权许可】

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