Somatic embryogenesis of Neoglaziovia variegata (Arruda) Mez, an important source of fiber from native Brazilian bromeliads

Brazilian Archives of Biology and Technology

Daniela Garcia Silveira²  Lucimeire Souza Morais Lino¹  Antônio Da Silva Souza¹  Fernanda Vidigal Duarte Souza¹ 

[1] ,Instituto Federal BaianoGuanambi BA ,Brasil

关键词: Caroá;    Bromeliaceae;    tissue culture;    2;    4-D;    glutamine;   

DOI  :  10.1590/S1516-89132013000400004

来源: SciELO

【 摘 要 】

Neoglaziovia variegata (Arruda) Mez, a Bromeliad endemic to the Brazilian Caatinga and source of fiber for many different products, is considered an endangered species. Leaf and stem segments were cultivated on Murashige and Skoog (MS) medium with combinations of 2,4- dichlorophenoxyacetic acid (2,4-D) (0, 9, 18 and 27 µM) and glutamine (0, 50, 100, 150, 200 mg L-1) for the induction of embryogenic calli. Leaf segments did not present a favorable response for the combinations analyzed. The best response for embryogenic calli frequency with the best distribution of somatic embryos on the surface and better conversion rate was obtained using the stems explants in the medium containing 18 µM 2,4-D and 100 mg L-1 glutamine. The embryogenic calli obtained from the different induction media were transferred to MS medium supplemented with 30 g.L-1 sucrose, 2.4 g.L-1 Phytagel®, 0.53 µM α-naphthalene acetic acid (NAA) and 0.88 µM of 6-benzylaminopurine (BAP). The regenerated plants presented normal growth; however, many embryos did not fully develop.

【 授权许可】

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