Analysis of seven STR human loci for paternity testing by microchip electrophoresis

Brazilian Archives of Biology and Technology

Karina Fraige¹  Regiane Fátima Travensolo¹  Emanuel Carrilho¹ 

[1] ,Universidade de São Paulo Instituto de Química de São Carlos São Carlos SP ,Brasil

关键词: Bioanalyzer 2100;    paternity testing;    polymerase chain reaction;    short tandem repeats;   

DOI  :  10.1590/S1516-89132013000200006

来源: SciELO

【 摘 要 】

The aim of this work was to evaluate two paternity cases by microchip electrophoresis and the validation of the methodology by comparison of the results with those obtained in a commercial genetic analyzer. It was observed that when working with tetranucleotide regions, in which the minimal difference between the alleles was only four base pairs, the commercial microchip system did not present the resolution and repeatability needed. Nevertheless, the relative standard deviation was between 0 and 1.2% and the fragments detected were within the expected size ranges as described in the literature.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License