Evaluation of oat hull hemicellulosic hydrolysate fermentability employing Pichia stipitis

Brazilian Archives of Biology and Technology

Luciana Cristina Silveira Chaud¹  Débora Danielle Virgínio Da Silva¹  Rafael Taino De Mattos¹  Maria Das Graças De Almeida Felipe¹ 

[1] ,Universidade de São Paulo Escola de Engenharia de Lorena Departamento de BiotecnologiaLorena SP ,Brasil

关键词: Pichia stipitis;    hemicellulosic hydrolysate;    oat hull;    ethanol;    activated charcoal;   

DOI  :  10.1590/S1516-89132012000500017

来源: SciELO

【 摘 要 】

Oat hull hemicellulosic hydrolysate obtained by diluted acid hydrolysis was employed as fermentation medium for Pichia stipitis cultivation. A comparison between the use of treated hydrolysate with 1% activated charcoal to reduce the toxic compounds generated during the hydrolysis process and untreated hydrolysate as a control was conducted. In the cultures using treated hydrolysate the total consumption of glucose, low xylose consumption and ethanol and glycerol formation were observed. The medium formulated with untreated hydrolysate showed morphological cell modifications with consequently cell death, no ethanol formation and formation of glycerol as byproduct of fermentative process, probably as a response to stressful conditions to yeast due to presence of high concentration of toxic compounds. Thus, further studies are suggested in order to determine the best conditions for hydrolysis and detoxification of the hydrolysate to improve the fermentative performance of P. stipitis.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License