In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

Brazilian Archives of Biology and Technology

David Delmail²  Pascal Labrousse¹  Jean-yves Lesouëf¹  François Le Hir¹  Jean-luc Autret¹  Michel Botineau¹ 

[1] ,University of Rennes1Rennes,France

关键词: Normania;    Endemic species;    Micropropagation;    6‑benzylaminopurine;    Callus;   

DOI  :  10.1590/S1516-89132012000400009

来源: SciELO

【 摘 要 】

Normania triphylla is an endemic species from Madeira island (Portugal) extinct in the wild since 1991. The aim of this work was to culture the meristems of this species in vitro and to multiply its shoots in order to preserve this endangered species. The best results in terms of multiplication were obtained in Murashige and Skoog medium supplemented with 10 µM 6‑benzylaminopurine (BAP). The number of shoots, the number of nodes and the number of leaves were the most important in this medium. However, the best results concerning the total shoot length were obtained when BAP was not supplemented into the medium and in the presence of 5 or 7.5 µM 1-naphtalene acetic acid (NAA). This is the first report on the in vitro culture of N. triphylla which could brings new avenues for the development of this species.

【 授权许可】

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